First-strand cDNA was synthesized from 1 g of total RNA using the iScriptTM cDNA Synthesis Package (Bio-Rad, Hercules, CA). LPS to improve GPR109A/HCA2 manifestation by … Continue reading First-strand cDNA was synthesized from 1 g of total RNA using the iScriptTM cDNA Synthesis Package (Bio-Rad, Hercules, CA)
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